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Static and dynamic imaging of alveoli using optical coherence tomography needle probes

McLaughlin, R.A., Yang, X., Quirk, B.C., Lorenser, D., Kirk, R.W., Noble, P.B. and Sampson, D.D. (2012) Static and dynamic imaging of alveoli using optical coherence tomography needle probes Journal of Applied Physiology, 113 (6). pp. 967-974.

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Imaging of alveoli in situ has for the most part been infeasible due to the high resolution required to discern individual alveoli and limited access to alveoli beneath the lung surface. In this study, we present a novel technique to image alveoli using optical coherence tomography (OCT). We propose the use of OCT needle probes, where the distal imaging probe has been miniaturized and encased within a hypodermic needle (as small as 30-gauge, outer diameter 310 μm), allowing insertion deep within the lung tissue with minimal tissue distortion. Such probes enable imaging at a resolution of ∼12 μm within a three-dimensional cylindrical field of view with diameter ~1.5 mmm centered on the needle tip. The imaging technique is demonstrated on excised lungs from three different species: adult rats, fetal sheep, and adult pigs. OCT needle probes were used to image alveoli, small bronchioles, and blood vessels, and results were matched to histological sections. We also present the first dynamic OCT images acquired with an OCT needle probe, allowing tracking of individual alveoli during simulated cyclical lung inflation and deflation.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
McLaughlin, R.A.
Yang, X.
Quirk, B.C.
Lorenser, D.
Kirk, R.W.
Noble, P.B.
Date : 2012
DOI : 10.1152/japplphysiol.00051.2012
Uncontrolled Keywords : Interstitial microscopy, Optical imaging, Optical pulmonary imaging, animal, article, blood vessel, bronchiole, computer assisted diagnosis, electronics, equipment design, histology, instrumentation, lung alveolus, needle, optical coherence tomography, prenatal development, rat, sheep, swine, three dimensional imaging, vascularization, Wistar rat, Animals, Blood Vessels, Bronchioles, Equipment Design, Histological Techniques, Image Interpretation, Computer-Assisted, Imaging, Three-Dimensional, Miniaturization, Needles, Pulmonary Alveoli, Rats, Rats, Wistar, Sheep, Swine, Tomography, Optical Coherence
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 05 Jun 2018 15:11
Last Modified : 19 Sep 2018 11:32

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