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Three-dimensional optical coherence tomography of whole-muscle autografts as a precursor to morphological assessment of muscular dystrophy in mice

Klyen, B.R., Armstrong, J.J., Adie, S.G., Radley, H.G., Grounds, M.D. and Sampson, David (2008) Three-dimensional optical coherence tomography of whole-muscle autografts as a precursor to morphological assessment of muscular dystrophy in mice Journal of Biomedical Optics, 13 (1).

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Three-dimensional optical coherence tomography (3D-OCT) is used to evaluate the structure and pathology of regenerating mouse skeletal muscle autografts for the first time. The death of myofibers with associated inflammation and subsequent new muscle formation in this graft model represents key features of necrosis and inflammation in the human disease Duchenne muscular dystrophy. We perform 3D-OCT imaging of excised autografts and compare OCT images with coregistered histology. The OCT images readily distinguish the necrotic and inflammatory tissue of the graft from the intact healthy muscle fibers in the underlying host tissue. These preliminary findings suggest that, with further development, 3D-OCT could be used as a tool for the evaluation of small-animal muscle morphology and pathology, in particular, for analysis of mouse models of muscular dystrophy.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
Klyen, B.R.
Armstrong, J.J.
Adie, S.G.
Radley, H.G.
Grounds, M.D.
Date : 2008
DOI : 10.1117/1.2870170
Uncontrolled Keywords : muscle, muscular dystrophy, optical coherence tomography, small-animal imaging, three-dimensional imaging, Coregistered, Further development, Graft-from, Host tissues, Human disease, Inflammatory tissues, Key feature, Mouse models, Muscle fiber, Muscular dystrophy, Myofibers, OCT imaging, Skeletal muscle, Small animal imaging, Small-animal, Three dimensional imaging, Diseases, Mammals, Muscle, Pathology, Tomography, Three dimensional, Animalia, Mus, algorithm, animal, article, C57BL mouse, computer assisted diagnosis, disease model, evaluation, feasibility study, female, graft rejection, image enhancement, methodology, mouse, muscular dystrophy, pathology, reproducibility, sensitivity and specificity, skeletal muscle, three dimensional imaging, transplantation, Algorithms, Animals, Disease Models, Animal, Feasibility Studies, Female, Graft Rejection, Image Enhancement, Image Interpretation, Computer-Assisted, Imaging, Three-Dimensional, Mice, Mice, Inbred C57BL, Muscle, Skeletal, Muscular Dystrophies, Reproducibility of Results, Sensitivity and Specificity
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 11 Jun 2018 07:33
Last Modified : 19 Sep 2018 11:32

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