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The biological fate of some chlorofluoroalkanes.

Cox, Peter John. (1972) The biological fate of some chlorofluoroalkanes. Doctoral thesis, University of Surrey (United Kingdom)..

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The literature on the toxicology and biochemical effects of chlorofluoroalkanes and closely related compounds of toxicological interest has been reviewed. The chlorofluoroalkanes used in aerosol propellant mixtures were the subject of this study. A simple, accurate quantitative g. l. c. assay was developed for the measurement of trichlorofluoromethane and 1,2-dichloro-1,1,2,2-tetrafluoroethane in extracts of tissues and the expired air. The separation and quantitation of mixtures of chlorofluoroalkanes was investigated. The distribution and excretion of trichlorofluoro-methane and 1,2-dichloro-1,1,2,2-tetrafluoroethane was determined after administration of these compounds by the oral route. Attempts were made to detect metabolism of trichloro-fluoromethane in in vitro incubations. Although it was feasible to detect 0.2% of a possible metabolite (dichloro-fluoromethane), no formation of this metabolite was detected in incubations of trichlorofluoromethane with microsomal preparations from several rodent species. Lipid peroxidation was investigated as a measure of free radical formation. Trichlorofluoromethane was found to have little effect on this parameter. Simple toxicological experiments showed the chlorofluoroalkanes to be relatively non-toxic even at very high dose levels. Total tissue lipid was not affected by trichlorofluoromethane. The chlorofluoroalkanes gave spectral interactions with cytochrome P-450, both in the oxidised and reduced states. The chlorofluoroalkanes did not affect type I substrate metabolism. The effects on type II substrate metabolism were inhibition (zoxazolamine hydroxylation) and activation (acetanilide and aniline 4-hydroxylations). The results are discussed in relation to the low toxicity of the chlorofluoroalkanes, and in comparison to the hepatotoxin, carbon tetrachloride.

Item Type: Thesis (Doctoral)
Divisions : Theses
Authors :
Cox, Peter John.
Date : 1972
Contributors :
Additional Information : Thesis (Ph.D.)--University of Surrey (United Kingdom), 1972.
Depositing User : EPrints Services
Date Deposited : 22 Jun 2018 13:00
Last Modified : 06 Nov 2018 16:52

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