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Measurement of Endothelium-Dependent Vasodilation in Mice--Brief Report

Schuler, D., Sansone, R., Freudenberger, T., Rodriguez-Mateos, A., Weber, G., Momma, T. Y., Goy, C., Altschmied, J., Haendeler, J., Fischer, J. W. , Kelm, M. and Heiss, C. (2014) Measurement of Endothelium-Dependent Vasodilation in Mice--Brief Report ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 34 (12). pp. 2651-2657.

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Objective —

Endothelium-dependent, flow-mediated vasodilation after an increase in shear stress at the endothelial lining of conduit arteries during reactive hyperemia after ischemia is a fundamental principle of vascular physiology adapting blood flow to demand of supplied tissue. Flow-mediated vasodilation measurements have been performed in human studies and are of diagnostic and prognostic importance, but have been impossible because of technical limitations in transgenic mice to date, although these represent the most frequently used animal model in cardiovascular research.

Approach and Results —

Using high-frequency ultrasound, we visualized, quantified, and characterized for the first time endothelium-dependent dilation of the femoral artery after temporal ischemia of the lower part of the hindlimb and demonstrated that the signaling was almost exclusively dependent on stimulation of endothelial nitric oxide synthase, similar to acetylcholine, completely abolished after pharmacological or genetic inhibition of endothelial nitric oxide synthase and endothelial denudation, substantially impaired in mice of increasing age and cholesterol-fed ApoE knock outs and increased by the dietary polyphenol (−)-epicatechin. Intra- and interindividual variability were similar to the human methodology.

Conclusions —

The physiology of flow-mediated vasodilation in mice resembles that in humans underscoring the significance of this novel technology to noninvasively, serially, and reliably quantify flow-mediated vasodilation in transgenic mice.—

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Clinical and Experimental Medicine
Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Faculty of Health and Medical Sciences
Authors :
Schuler, D.
Sansone, R.
Freudenberger, T.
Rodriguez-Mateos, A.
Weber, G.
Momma, T. Y.
Goy, C.
Altschmied, J.
Haendeler, J.
Fischer, J. W.
Kelm, M.
Date : 23 October 2014
DOI : 10.1161/ATVBAHA.114.304699
Copyright Disclaimer : © 2014 American Heart Association, Inc.
Uncontrolled Keywords : Endothelial nitric oxide synthase; Femoral artery; Vasodilation; Flavonoids; Blood flow velocity; Aging; Doppler duplex ultrasonography
Depositing User : Diane Maxfield
Date Deposited : 20 Aug 2018 10:45
Last Modified : 20 Aug 2018 10:45

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